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is there a limit in the number of IOL nodes that I can run (Assuming that I have the necessary resources)?

I can successfully run 63 IOL nodes but node number 64 is unable to start. I can add any other node, vIOS or ASAv, after my 63rd IOL node and they will start successfully. if there is a limit, is there a way to bypass it?

64 is limit for IOL, because of MAC address collisions after

Yes a limitation exist. Due to IOL mac address distribution. Mac depend of IOL ID.

IOL ID is limited to 1024 . ( 10 bits value )

Eve use id to avoid mac collision like this:
4 bits are used for User ID ( 0 -> 15 )
6 bits are used for Node ID ( 0->63 )

This permit to have multiple time same lab using a IOL connected to pnet0 without mac duplicate...

So EVE cannot support more than 63 IOL ID. The origin of this limitation is due IOL Mac compute algorithm...

E.

PS: Sorry to be so technical...